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目的探讨绝经后妇女血清成骨细胞分泌的基质金属蛋白酶1、2(MMP-1,MMP-2)和组织金属蛋白酶抑制因子1(TIMP1)与骨密度及骨转换生化指标之间的关系。方法用酶联免疫吸附试验测定297名48～80岁女性志愿者的血清MMP-1、MMP-2、TIMP-1、血清骨碱性磷酸酶(BAP)、血清骨钙素和血清Ⅰ型胶原氨基末端肽(NTX),用双能量X射线吸收测定术测定腰椎正位、L1L4总体、股骨颈、Ward′s区、总髋部的骨密度。结果(1)绝经后妇女血清MMP-1、TIMP-1水平与骨密度、骨转换生化指标无明显相关性。(2)血清MMP-2水平与骨密度呈较弱的负相关,校正年龄与体重指数后,与股骨颈、髋部骨密度的相关性消失。(3)MMP2与BAP、骨钙素、NTX正相关(相关系数分别为0.193、0.231、0.249,均P<0.01)。(4)绝经后骨质疏松症患者血清MMP2水平(1466μg/L±313μg/L)高于年龄匹配的正常对照组(1222μg/L±243μg/L)、低骨量组(1282μg/L±220μg/L)(P<0.01)。结论绝经后妇女血清MMP-2与骨转换生化指标相关联,血清MMP-2水平升高可能为高骨代谢转换过程(如绝经后骨质疏松症)中的一种伴随表现。
Objective To investigate the relationship between the expression of matrix metalloproteinase-1 (MMP-2), tissue inhibitor of metalloproteinase-1 (TIMP1) and bone mineral density (BMD) and biochemical markers of bone turnover in postmenopausal women. Methods Serum levels of MMP-1, MMP-2, TIMP-1, BAP, serum osteocalcin and serum type I collagen in 297 female volunteers aged 48 to 80 years were measured by enzyme-linked immunosorbent assay Amino-terminal peptide (NTX) was measured by dual-energy X-ray absorptiometry in the lumbar spine, L1L4 population, femoral neck, Ward’s area and total hip. Results (1) Serum levels of MMP-1 and TIMP-1 in postmenopausal women had no significant correlation with bone mineral density and biochemical markers of bone turnover. (2) There was a weak negative correlation between serum MMP-2 level and BMD. Correlation of age and BMI with the BMD of femoral neck and hip disappeared. (3) There was a positive correlation between MMP2 and BAP, osteocalcin and NTX (correlation coefficients were 0.193,0.231,0.249, all P <0.01). (4) Serum levels of MMP2 in postmenopausal osteoporosis patients (1466μg / L ± 313μg / L) were significantly higher than those in age-matched normal controls (1222μg / L ± 243μg / L) /L)(P<0.01). Conclusions Serum MMP-2 is associated with biochemical markers of bone turnover in postmenopausal women. Increased serum MMP-2 levels may be a concomitant manifestation of metabolic processes such as postmenopausal osteoporosis.