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目的探讨神经调节蛋白1(NRG1)对人冠状动脉平滑肌细胞(HCASMC)增殖和迁移的影响。方法培养HCASMC,Western blot法检测HCASMC中表皮生长因子受体2(Erb B2)、Erb B3和Erb B4的表达以及Erb B2、Erb B3和Erb B4的磷酸化;MTT法检测不同浓度NRG1刺激对HCASMC增殖的影响,利用TranswellTM小室检测不同浓度NRG1刺激对HCASMC迁移能力的影响。结果 Erb B2、Erb B3和Erb B4均能在HCASMC中表达,加入NRG1处理后,与空白对照组比较,3个受体磷酸化水平均明显增加。不同浓度的NRG1干预对HCASMC增殖和迁移的影响不同,10 ng/m L的NRG1能明显促进HCASMC的增殖和迁移。结论 NRG1通过增强其受体Erb B2、Erb B3和Erb B4的磷酸化促进HCASMC的增殖和迁移,进而可能促进HCASMC在血管生成中的作用。
Objective To investigate the effects of neuregulin 1 (NRG1) on the proliferation and migration of human coronary artery smooth muscle cells (HCASMC). Methods HCASMC were cultured and the expression of Erb B2, Erb B3 and Erb B4 and the phosphorylation of Erb B2, Erb B3 and Erb B4 in HCASMC were detected by Western blot. The effects of different concentrations of NRG1 stimulation on the expression of HCASMC Proliferation, the use of TranswellTM chamber detection of different concentrations of NRG1 stimulation HCASMC migration. Results Erb B2, Erb B3 and Erb B4 were all expressed in HCASMC. After adding NRG1, the phosphorylation levels of three receptors were significantly increased compared with the blank control group. The effects of different concentrations of NRG1 on the proliferation and migration of HCASMC were different. NRG1 at 10 ng / mL could significantly promote the proliferation and migration of HCASMC. Conclusion NRG1 can promote the proliferation and migration of HCASMC by enhancing the phosphorylation of its receptor Erb B2, Erb B3 and Erb B4, which may further promote the role of HCASMC in angiogenesis.