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试验结果表明ＭＳ、１／２ＭＳ、ＫＣ、ＶＷ四种培养基对大花蕙兰（Ｃｙｍｂｉｄｉｕｍ）原球茎增殖倍数没有明显影响，但其发芽率与生根率表现各异。原球茎增殖培养基中加入０．５ｍｇ／ＬＮＡＡ＋１ｍｇ／１Ａｄ有利于原球茎增殖倍数的提高，但降低生根率。单独施用０．５ｍｇ／ＬＮＡＡ明显提高原球茎增殖倍数与生根率。四种不同细胞分裂素处理中，以１ｍｇ／ＬＢＡ最有利于原球茎增殖倍数的提高。２５℃较２０℃有利于原球茎增殖。井字型切割缩短继代培养周期，有利于原球茎增殖周期，有利于原球茎增殖。大花蕙兰试管苗在采用一般炼苗方法可获得９５％以上的移栽成活率。
The results showed that MS, 1 / 2MS, KC and VW had no significant effect on the multiplication of cytoplasm of Cymbidium, but their germination rate and rooting rate varied. The addition of 0.5mg / L NAA + 1mg / 1Ad to protocorm multiplication medium was beneficial to the multiplication of protocorm multiplication, but decreased the rate of rooting. Administration of 0.5 mg / L NAA alone significantly increased protocorm multiplication ratio and rooting rate. Of the four different cytokinin treatments, 1 mg / L BA most favored multiplication of protocorm multiplication. 25 ℃ than 20 ℃ conducive to protocorm proliferation. Tic-tac-cutting to shorten the subculture period is conducive to the protocorm multiplication cycle, is conducive to protocorm multiplication. Cymbidium test tube seedlings in the general method of refining can be obtained more than 95% survival rate of transplanting.