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目的研究伴重症肌无力(MG)胸腺瘤患者胸腺瘤中天然调节性T细胞(nTreg)的变化及其影响因素。方法采用免疫组化方法检测不同病理类型胸腺瘤中nTreg细胞的数量,通过实时定量反转录PCR检测不同病理类型胸腺瘤中FoxP3 mRNA及胸腺间质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)mRNA水平,并分析二者之间的关系。结果伴MG胸腺瘤中FoxP3+Treg细胞数量较正常胸腺组织减少,差异有统计学意义(P<0.01);伴MG胸腺瘤FoxP3 mRNA、TSLP mRNA转录水平较正常胸腺组织明显降低,差异有统计学意义(P<0.01);在各胸腺瘤亚型中,B1型FoxP3+Treg细胞数量,FoxP3 mRNA、TSLP mRNA转录水平最高;FoxP3 mRNA、TSLP mRNA水平呈显著正相关。结论伴MG胸腺瘤中nTreg减少,与胸腺瘤中TSLP转录水平下降有关,可能导致免疫调节功能紊乱,从而引起MG发病。
Objective To study the changes of natural regulatory T cells (nTregs) in thymoma patients with myasthenia gravis (MG) and its influencing factors. Methods Immunohistochemistry was used to detect the number of nTreg cells in different pathological types of thymoma. Foxp3 mRNA and thymic stromal lymphopoietin (TSLP) mRNA were detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) in different pathological types of thymoma Level, and analyze the relationship between the two. Results Compared with normal thymus, the number of FoxP3 + Treg cells in MG thymoma was significantly lower than that in normal thymus (P <0.01). The transcription levels of FoxP3 mRNA and TSLP mRNA in MG thymoma were significantly lower than those in normal thymus (P <0.01). The number of Foxp3 + Treg cells, FoxP3 mRNA and TSLP mRNA were the highest in all thymoma subtypes, and Foxp3 mRNA and TSLP mRNA were positively correlated with each other. Conclusions The decrease of nTreg in MG thymoma is related to the decrease of TSLP transcription level in thymoma, which may result in the disorder of immune regulation and lead to the onset of MG.