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目的 探讨 3p、9p微卫星分析在口腔粘膜癌前病变中的改变及应用价值。 方法 应用PCR为基础的微卫星分析技术 ,选取人正常口腔粘膜 (8例 )、口腔白斑 (31例 )及口腔鳞状细胞癌 (2 2例 )标本 ,分析分别位于3p(D3S6 5 9)、9p(D9S16 1、D9S15 7、D9S171)上四位点微卫星的改变。 结果 在正常口腔粘膜 (NOM )及单纯上皮增生组均未见等位基因的改变。在口腔鳞癌 (OSCC)组中 12 / 2 2 (5 4.5 % )存在至少一个位点的杂合性缺失 (LOH) ,异常增生组中 6 / 2 3(2 5 .1% )存在至少一个位点的LOH。还观察到这几个位点均存在一定频率的微卫星不稳定性(MI)。OSCC组 9/ 2 2观察到MI,达 40 .9% ,异常增生组中达 2 1.9%。结论 3p、9p上可能存在与口腔鳞癌相关的抑癌基因。 3p、9p上这四位点的改变可能系口腔鳞癌发生的早期分子事件。本研究提示该四位点微卫星不稳定性在一部分口腔癌发生中起一定作用。
Objective To investigate the changes and application value of 3p, 9p microsatellite analysis in oral mucosal precancerous lesions. Methods The specimens of normal oral mucosa (8 cases), oral leukoplakia (31 cases) and oral squamous cell carcinoma (22 cases) were selected by PCR-based microsatellite analysis. 9p (D9S16 1, D9S15 7, D9S171) on the four-site microsatellite changes. Results There was no allele change in normal oral mucosa (NOM) and simple epithelial hyperplasia group. In the OSCC group, there were at least one locus heterozygosity deletion (LOH) in 12/2 2 (4.5%) and in 6/23 (25.1%) in the dysplasia group there was at least one LOH of the locus. A few frequencies of microsatellite instability (MI) were also observed at these sites. In the OSCC group 9/2 2 MI was observed, reaching 40.9% and in the group of abnormal proliferation reaching 2 1.9%. Conclusion 3p, 9p may exist on oral squamous cell carcinoma suppressor gene. The changes of these four sites on 3p and 9p may be the early molecular events of oral squamous cell carcinoma. This study suggests that the four-site microsatellite instability plays a role in some oral carcinogenesis.