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目的:研究反应物浓度、反应时间及pH值等因素对羟胺缩合紫外分光光度法测戊二醛的吸收光谱的影响,分析光谱特征及其产生的原因;方法:配制不同pH值的溶液作为稀释液,取不同比例的盐酸羟胺和戊二醛分别以各稀释液稀释至一定体积进行反应,在10、30、50、分钟时分别扫描各反应溶液的紫外吸收光谱图进行比较;结果:反应液在255nm～260nm之间的光谱比较平缓,吸收度远远高于同波长下的戊二醛溶液;在酸性及中性环境下,溶液的吸收光谱随着反应时间的延长向右移动,而在碱性环境下,光谱不随时间改变;当羟胺浓度增加时,吸收光谱左移;结论:在pH=11的碱性环境下,以1:2摩尔比例反应约20分钟,在256nm处进行测定,重复性好、结果最稳定。
OBJECTIVE: To study the influence of reactant concentration, reaction time and pH value on the absorption spectrum of glutaraldehyde by UV-A condensation UV spectrophotometry and to analyze the characteristics of the spectrum and the reasons for its occurrence. Methods: Different pH solutions were prepared as dilution Liquid, take different proportions of hydroxylamine hydrochloride and glutaraldehyde were diluted with each dilution to a certain volume of the reaction at 10,30,50 minutes, respectively, when scanning the reaction solution UV absorption spectra were compared; results: the reaction solution The spectrum between 255nm and 260nm is relatively flat and the absorbance is much higher than that of the glutaraldehyde solution at the same wavelength. Under acidic and neutral conditions, the absorption spectrum of the solution moves to the right with the extension of the reaction time, Under alkaline environment, the spectrum does not change with time; when the concentration of hydroxylamine increases, the absorption spectrum shifts to the left; Conclusion: The reaction is carried out at a molar ratio of 1: 2 in alkaline environment at pH = 11 for about 20 minutes and at 256 nm, Repeatability, the result is the most stable.