论文部分内容阅读
目的:利用HPLC法同时测定通宣理肺丸中橙皮苷与柚皮苷的含量。方法:以C18键合硅胶为填充剂,甲醇-醋酸-水(35∶4∶61)为流动相,检测波长283 nm,流速1.0mL.min-1。结果:橙皮苷在进样量0.085～1.7μg范围内,呈良好的线性关系Y=3 145.5 X-36.594(r=0.999 7),平均回收率为99.03%,RSD为1.59%(n=6);柚皮苷在进样量0.063～1.26μg范围内,呈良好的线性关系Y=2 630.8 X+73.163(r=0.999 1);平均回收率为98.76%,RSD为0.97%(n=6)。结论:本法快速、准确,简便,重复性良好,可用于测定通宣理肺丸中橙皮苷、柚皮苷的含量控制。
Objective: To determine the content of hesperidin and naringin in Tongxuanlifei Pills simultaneously by HPLC method. Methods: C18 bonded silica gel was used as the filler, and methanol-acetic acid-water (35:4:61) was used as the mobile phase. The detection wavelength was 283 nm and the flow rate was 1.0 mL · min-1. Results: Hesperidin showed a good linear relationship in the range of 0.085-1.7 μg of sample volume (Y = 3 145.5 X-36.594, r = 0.999 7) with an average recovery of 99.03% and RSD of 1.59% (n = 6 ); Naringin showed a good linear relationship in the range of 0.063-1.26 μg, with an average recovery rate of 98.76% and a mean RSD of 0.97% (n = 6 ). Conclusion: The method is rapid, accurate, simple and reproducible. It can be used to determine the content of hesperidin and naringin in Tongxuolifei pill.