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目的通过对鼻硬结杆菌5种特殊染色方法的观察,寻找显示鼻硬结杆菌的最佳方法,以提高其病理诊断水平。方法收集45例鼻硬结病患者的临床病理资料,以改良沃森-斯塔里(Warthin-Starry,W-S)、过磺酸雪夫(PAS)、美蓝、姬姆萨(Giemsa)、革兰氏染色方法显示鼻硬结杆菌,比较其染色结果。结果5种染色方法均能显示吞噬细胞内的鼻硬结杆菌。W-S染色菌体呈黑色、短棒状,组织染成棕黄色,对比清晰,染色效果最佳;PAS、美蓝、Giemsa及革兰氏染色菌体轮廓均较为清晰,但组织背景着色与菌体颜色较为接近,对比效果较差。结论5种染色均可显示鼻硬结杆菌,W-S染色效果最佳,可为鼻硬结病的病理诊断提供确定的病原学依据。
OBJECTIVE To find out the best method for the identification of Corynebacterium nosocomialum by observing five special staining methods of Corynebacterium nosocomialum in order to improve its pathological diagnosis. Methods The clinicopathological data of 45 patients with nasal cirrhosis were collected to improve Warthin-Starry (WS), PAS, Methylene blue, Giemsa, Staining method shows Corynebacterium nosocomial, compared with the staining results. Results All five staining methods showed that the Amycolatopsis niloticus in phagocytic cells. WS stained black, short rod-shaped, tissue stained brown yellow, clear contrast, the best staining; PAS, methylene blue, Giemsa and Gram stain the outline of the bacteria are more clear, but the background staining and cell body color More close, the comparison is less effective. Conclusion All the five stains can be used to display the positive results of W-S stain, which can provide a definite etiological basis for the pathological diagnosis of rhinosclerosis.