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目的:观察急性和慢性再障患者联合免疫疗法治疗前、后外周血和骨髓中白细胞介素8(IL-8)的含量变化和临床意义,并探讨其对监测再障疗效的可行性。方法:应用实时定量PCR和ELISA法检测外周血和骨髓IL-8的含量,流式细胞术分析外周血中CD4/CD8阳性细胞比值变化。结果:急性和慢性再障患者外周血和骨髓中IL-8含量均远高于正常对照者,且CD4/CD8出现倒置。抗淋巴细胞球蛋白(ALG)加环孢素A(CsA)加雄激素较CsA加雄激素治疗效果明显,总有效率分别为83.3%和62.5%。ALG加CsA加雄激素治疗重症再障较CsA加雄激素疗效显著。细胞因子水平改变和疗效明显相关。实时定量PCR和ELISA法正相关,但前者较易测出和量化。结论:ALG联合CsA治疗急、慢性再障疗效显著。细胞因子IL-8可以作为急、慢性再障的疗效监测指标。
OBJECTIVE: To observe the changes and clinical significance of interleukin-8 (IL-8) levels in peripheral blood and bone marrow of patients with acute or chronic aplastic anemia before and after treatment, and to explore its feasibility of monitoring the efficacy of aplastic anemia. Methods: The contents of IL-8 in peripheral blood and bone marrow were detected by real-time PCR and ELISA. The ratio of CD4 / CD8 positive cells in peripheral blood was analyzed by flow cytometry. Results: The levels of IL-8 in peripheral blood and bone marrow of patients with acute and chronic aplastic anemia were much higher than those of normal controls, and CD4 / CD8 appeared upside down. Anti-lymphocyte globulin (ALG) plus cyclosporin A (CsA) plus androgen than CsA plus androgen treatment effect is obvious, the total effective rate was 83.3% and 62.5%. ALG plus CsA plus androgen treatment of severe aplastic anemia than CsA plus androgen significant effect. Changes in cytokine levels and efficacy were significantly correlated. Real-time quantitative PCR and ELISA are positively correlated, but the former is easier to measure and quantify. Conclusion: ALG combined with CsA treatment of acute and chronic aplastic anemia significant effect. Cytokine IL-8 can be used as an indicator of the efficacy of acute and chronic aplastic anemia.