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目的:检测HHV-6、7、8及CMV在大肠肿瘤中的感染,探讨其致病的协同作用。方法:取20例大肠肿瘤标本,其中10例良性病变(炎性息肉、乳头状瘤和绒毛状腺瘤),10例恶性病变(癌和腺瘤恶变)。应用巢式PCR法,分别检测两组病变组织中HHV-6、7、8和MCV的DNA片段。结果:HHV-6检测结果表明恶性病变组阳性反应率为90%(9/10),良性病变组阳性反应率为60%(6/10),其产物为132bpDNA片段;HHV-7检测结果示恶性病变组阳性反应率为40%(4/10),良性病变组阳性反应率为0(0/10),其产物为92bpDNA片段;HHV-8检测结果示恶性病变组阳性反应率为90%(9/10),良性病变组阳性反应率为20%(2/10),其产物为177bpDNA片段;检测CMV结果示恶性病变样本组阳性反应率为100%(10/10),良性病变样本组阳性反应率为100%(10/10),其产物为85bpDNA片段。结论:CMV在良、恶性大肠肿瘤病变中均有较高的感染率,HHV-6、7、8在恶性病变组中感染率较良性组高,是需要警惕的致病因素。
Objective: To detect the infection of HHV-6, 7, 8 and CMV in colorectal tumor and to explore their synergistic effect. Methods: Twenty samples of colorectal tumors were collected, including 10 benign lesions (inflammatory polyp, papilloma and villous adenoma) and 10 malignant lesions (malignant transformation of adenoma and adenoma). Nested PCR was used to detect the DNA fragments of HHV-6, 7, 8 and MCV in the diseased tissues of two groups. Results: The positive rate of HHV-6 was 90% (9/10) in malignant group and 60% (6/10) in benign group. The result of HHV-7 showed a 132bp DNA fragment The positive reaction rate was 40% (4/10) in malignant group and 0 (0/10) in benign group, and the product was 92bp DNA fragment. The positive rate of HHV-8 in test group was 90% (9/10). The positive rate of benign lesion group was 20% (2/10) and the product was 177 bp. The positive rate of CMV in the malignant lesion group was 100% (10/10) Group positive reaction rate was 100% (10/10), the product of 85bp DNA fragment. CONCLUSION: CMV has a high infection rate in both benign and malignant colorectal neoplasms. The infection rate of HHV-6, 7 and 8 in malignant neoplasm group is higher than that of benign group, which is the risk factor to be vigilant.