Development and Preliminary Application of Double Antibody Sandwich ELISA for Detection of Swine Ves

• 作 者: WU Guo-hua YAN Xin-min ZHAO Zh
• 机 构: StateKeyLaboratoryofVeterinaryEtiologicalBiology
• 出 处: 动物与饲料科学：英文版
• 发表日期: 2011年4期
• 关键词: ELISA检测 猪水泡病病毒 双抗体夹心 酶联免疫吸附试验 酶联免疫吸附检测 世界动物卫生组织 ELISA法 标准样品 Swine vesicular dise
• 基金项目: This work was funded by the Major Sci-Tech Fund of Gansu Province （092NKDA032）.

[Objective] The aim of this study was to develop an indirect sandwich ELISA method for detection of swine vesicular disease virus (SVDV). [Method] High titer serum against SVDV was prepared respectively by inoculated rabbits and guinea pigs with purified