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目的探讨103钯(103pd)放射性支架对p53抑癌基因在辐射诱导胆管癌细胞凋亡中的影响和意义。方法 12只裸鼠随机分2组,每组6只,在裸鼠胆管癌内分别植入103pd放射性支架和普通胆管支架,术后30 d取出胆管癌标本进行HE染色和p53基因原位杂交,DAB染色p53基因表达阳性者细胞质呈棕黄色,胆管癌细胞凋亡染色细胞核中有棕黄色浓染的为阳性细胞。结果103pd放射性支架组胆管癌细胞p53基因阳性表达率为89.63%,而普通支架组为21.76%,2组比较差异有统计学意义(P<0.01);103pd放射性支架组胆管癌凋亡细胞数明显大于普通支架组,2组比较差异有统计学意义(P<0.05)。结论103pd放射性支架可以激活p53基因,使其功能提高,诱导胆管癌细胞凋亡。
Objective To investigate the effect of 103 Pd (103pd) radioactive stent on the apoptosis of p53 cholangiocarcinoma cells induced by radiation. Methods Twelve nude mice were randomly divided into two groups with 6 mice in each group. 103pd radioactive stents and common biliary stents were implanted in nude mice respectively. Thirty days later, the specimens of cholangiocarcinoma were removed for HE staining and p53 gene in situ hybridization. DAB staining p53 gene positive cytoplasm was brown, cholangiocarcinoma cells stained nuclei brownish yellow staining for positive cells. Results The positive rate of p53 gene expression in 103pd group was 89.63%, which was 21.76% in common scaffold group, the difference was statistically significant (P <0.01). The number of apoptotic cells in 103pd group was significantly higher than that in 103pd group Compared with the normal stent group, the difference between the two groups was statistically significant (P <0.05). Conclusion 103pd radioactive scaffold can activate p53 gene to enhance its function and induce apoptosis of cholangiocarcinoma cells.