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目的分析宝鸡地区的汉坦病毒基因序列特征。方法收集肾综合征出血热患者血清及汉坦病毒宿主动物鼠肺标本,通过荧光PCR方法进行基因分型,采用RT-PCR对目的基因进行扩增并测序,与汉坦病毒分型菌株进行序列比对及种系发生树分析。结果宝鸡地区汉坦病毒分型检测结果均为汉滩型(HTN),未检测到汉城型(SEO);序列分析显示,获得的11株序列与宁夏分离株Niongxia-A及陕西分离株A16同源性为94.3%~97.0%,同属于汉滩型H9亚型;1株序列与其他11株序列同源性仅为76.5%~77.4%,进一步序列比对显示与Nc167毒株同源性较高。结论宝鸡地区汉坦病毒基因型为汉滩型H9亚型;检测到1株与大别山病毒(DBSV)高度同源,不排除存在其他汉坦病毒型别的可能。
Objective To analyze the sequence of Hantavirus gene in Baoji area. Methods The serum samples from haemorrhagic fever with renal syndrome and Hantaan virus infected host mice were collected and genotyped by fluorescent PCR. The target gene was amplified and sequenced by RT-PCR and sequenced with Hantavirus strain Alignment and phylogenetic tree analysis. Results The genotypes of Hantavirus in Baoji were all Hantaan type (HTN) and no SEO was detected. Sequence analysis showed that the 11 sequences obtained were identical with those of Ningxia isolate Niongxia-A and Shaanxi isolate A16 The homology was from Hantaan type H9 subtypes with 94.3% -97.0%, and the homology between one strain and other 11 strains was only 76.5% -77.4%. Further sequence alignment showed that the homology was higher than that of Nc167 strain high. Conclusion The genotype of Hantaan virus in Baoji region is Hantaan type H9 subtype. One strain of Hantaan virus was found to be highly homologous to Dabashan virus (DBSV), not to exclude the possibility of other Hantavirus genotypes.