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通过高效液相色谱法研究了黄芩苷在大鼠胃、离体小肠的吸收情况，采用ＮＵＣＬｅｏｓｉｌＣ１８（５ μｍ）４ ｍ ｍ ×２５０ ｍ ｍ 色谱柱，以甲醇水乙酸（６０∶３９∶１） 为流动相，检测波长２７６ ｎｍ ，柱温：室温，本实验对大鼠胃和离体小肠在不同ｐＨ 条件下的吸收动力学进行了研究，实验结果：大鼠胃的吸收率与黄芩苷的油／ 水分配系数之间相关方程为：吸收率＝ ０ ．０３０ ５７１ ｇ（ 油／ 水分配系数） － ２ ．１２６（ｒ＝ ０－９７３１） ；大鼠离体小肠的吸收率与黄芩苷的油／ 水分配系数之间相关方程为：吸收率＝０－０８４ １ ×１ ｇ（ 油／ 水分配系数） － ３－２５４（ｒ＝ ０．９９６ ２） ．
The absorption of baicalin in rat stomach and isolated small intestine was studied by HPLC, using NUCLeosilC18 (5 μm) 4 m m ×250 m m column with methanolwateracetic acid (60∶39). 1:1) is the mobile phase, the detection wavelength is 276 nm, the column temperature is room temperature, and the absorption kinetics of rat stomach and isolated small intestine under different pH conditions are studied in this experiment. Experimental results: rat stomach absorption rate The correlation equation between the oil/water partition coefficients of baicalin is: Absorptivity = 0 . 030 571 g (oil/water partition coefficient) - 2. 126 (r = 0-9731); The correlation equation between the absorption rate of rat isolated small intestine and the oil/water partition coefficient of baicalin is: Absorptivity = 0-084 1 × 1 g (oil/water partition coefficient) - 3-254 (r = 0.996 2).