论文部分内容阅读
本文采用双层软琼脂培养法探讨HeLa细胞集落形成的适宜条件为,含20%小牛血清、20%HeLa-cs的RPMI1640培养液及35mm×10mm培养皿中接种的细胞浓度为5×10~3。
The suitable conditions for the formation of HeLa cells colony using double-layer soft agar culture method is that the concentration of cells inoculated with 20% calf serum, 20% HeLa-cs in RPMI1640 medium and 35mm×10mm petri dish is 5×10~ 3.