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乙烯参与植物生长发育等许多生理过程,在许多植物细胞中,ACO基因通过促进乙烯合成调控着植物器官的形成发育。研究通过PCR方法从棉花基因组中克隆得到1-氨基环丙烷-1-羧酸氧化酶1(GhACO1)基因,并将该基因构建到植物表达载体PBI121中,其中GhACO1基因位于CaMV35S启动子和GUS报告基因之间。通过花滴法将GhACO1基因转化拟南芥,利用卡那霉素对转化植株进行初步筛选,对卡那霉素阳性植株进行进一步的PCR检测和GUS组织化学分析。结果表明:GhACO1基因已经整合到拟南芥基因组中;GUS组织化学分析显示,在转基因拟南芥叶、茎和根中都表现出GUS活性。研究结果为进一步探讨GhACO1的生物学功能和基因工程改良棉花纤维品质奠定了基础。
Ethylene participates in many physiological processes such as plant growth and development. In many plant cells, ACO gene regulates the formation and development of plant organs by promoting ethylene synthesis. In this study, 1-aminocyclopropane-1-carboxylate oxidase 1 (GhACO1) gene was cloned from the cotton genome by PCR and constructed into plant expression vector PBI121. The GhACO1 gene was located in CaMV35S promoter and GUS reporter Between genes. The GhACO1 gene was transformed into Arabidopsis thaliana by floral dripping method. The kanamycin-producing plants were initially screened for kanamycin-positive plants for further PCR detection and GUS histochemical analysis. The results showed that the GhACO1 gene was integrated into Arabidopsis thaliana genome. GUS histochemical analysis showed GUS activity in transgenic Arabidopsis leaves, stems and roots. The results laid the foundation for the further study on the biological function of GhACO1 and genetic improvement of cotton fiber quality.