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The structure, function and working principle of JLUIV-3, which is a new type of automated guided vehicle (AGV) with computer vision, is described.The white stripe line with certain width is used as inductive mark for JLUIV-3 automated navigation.JULIV-3 can automatically recognize the Arabic numeral codes which mark the multi-branch paths and multi-operation buffers, and autonomously select the correct path for destination.Compared with the traditional AGV, it has much more navigation flexibility and less cost, and provides higher-level intelligence.The identification method of navigation path by using neural network and the optimal control method of the AGV are introduced in detail.