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以美国国家航空航天局(NASA)提供的经空间飞行处理(SpaceExposedSed,SES)和地面留存(EarthBasedSed,EBS)的番茄种子为材料,测定了空间飞行处理后番茄种子活力变化及其与活性氧代谢的关系。结果表明:SES发芽势、发芽率分别比EBS高8.8%和9.8%;在种子萌发过程中,可溶性蛋白质含量和O-·2含量逐渐下降,SOD活性则逐渐升高,但SES比EBS具有较高的蛋白质含量和SOD活性以及代谢强度,尤其是萌发初期两者差异更明显。空间飞行处理后,萌发9d的幼苗体内SOD、POD、和GSH-Px活性显著高于对照,而O-·2含量和MDA含量则显著低于对照。表明空间飞行处理提高番茄种子活力和促进初期生长与其提高种子及幼苗体内活性氧防御酶系统的活性、增强种子抗氧化能力、延缓种子衰老有关。
Tomato seeds from space flight treatment (SES) and earth-based reservation (EBS) provided by NASA were used to study the changes of tomato seed vigor after space flight treatment and its relationship with active oxygen metabolism Relationship. The results showed that the germination potential and germination rate of SES were 8.8% and 9.8% higher than those of EBS respectively. During the germination process, soluble protein content and O- · 2 content decreased gradually while SOD activity increased gradually. However, SES The difference between EBS with higher protein content and SOD activity and metabolic intensity, especially in the early germination stage, was more obvious. After space flight, the activities of SOD, POD and GSH-Px in germination 9d seedlings were significantly higher than those in the control, while the contents of O · 2 and MDA were significantly lower than those of the control. The results showed that space flight treatment can improve tomato seed vigor and promote initial growth, which is related to increasing activity of reactive oxygen species defense enzyme system in seeds and seedlings, enhancing seed antioxidant capacity and slowing seed aging.