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目的:对卫矛科植物雷公藤Tripterygium wilfordii Hook.F的干燥根茎进行固态生物转化后的化学成分进行了初步研究。方法:雷公藤生物转化产物用甲醇提取,依次用石油醚、氯仿、醋酸乙酯、正丁醇萃取,对氯仿部分采用各种柱色谱进行分离纯化,纯化物通过波谱数据分析进行结构鉴定。结果:从具有药理活性的氯仿部分分离到5个化合物,分别鉴定为:Caffeine(1),82-dienabieta-ll,14-dione-19-acid(2),β-Sitosterol(3),Stigmastero1(4),3β-Hydroxy-5α,8α-epidioxyergosta-6,22-diene(5),而雷公藤的主要毒性二萜成分雷公藤甲素和雷公藤内酯却没有被发现。结论:雷公藤的主要毒性二萜成分雷公藤甲素和雷公藤内酯却没有从活性部位氯仿萃取物中分到,证明转化脱毒是有意义的。
OBJECTIVE: To study the chemical constituents of the dried rhizomes of the Ceratophyllum Tripterygium wilfordii Hook.F after solid-state biotransformation. Methods: The biotransformation of Tripterygium wilfordii was extracted with methanol, followed by extraction with petroleum ether, chloroform, ethyl acetate and n-butanol. The chloroform fractions were separated and purified by various column chromatography. The purified compounds were identified by spectral data analysis. Results: Five compounds were isolated from the chloroform fractions with pharmacological activities and identified as Caffeine (1), 82-dienabieta-ll, 14-dione- 4), 3β-Hydroxy-5α, 8α-epidioxyergosta-6,22-diene (5), while triptolide and triptolide, the major toxicant of Tripterygium wilfordii, were not found. CONCLUSION: Triptolide, the main toxic diterpene triptolide of Tripterygium wilfordii, and triptolide are not fractionated from the chloroform extract of the active site. It is of great significance to prove that transformation is detoxification.