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在原种生产过程中,有时由于培养料过干,pH不当,营养元素缺乏等原因,导致菌丝发菌慢,甚至不吃料的现象,如果任其发展下去,必然会产生劣质菌种,返工又会造成严重损失。为了解决这一问题,特向广大菇友介绍一种补充液的添加方法。 (一)补充液的配制 如果原种仅因缺水原因,只需补用自来水或浇清洁塘水即可;若pH过低,可加澄清石灰水或添加少量NaOH,溶液碱性应低于pH9;若pH值过高,可添加适量盐酸,pH值应大于5;营养方面需要什么补充什么。 (二)补充液的灭菌 将补充液盛于大瓷盆或其它适宜容器中,内放勺子,上盖牛皮纸或耐压塑料,用橡皮筋或绳扎紧,盖上盖子,放入高压锅内,在1.4kg/cm~2压力下灭菌10～20分钟,或常压灭菌30～40分钟。冷却至30℃以下待用。 (三)补充液的添加 将原种、补充液、镊子,酒精灯、火柴等放入接种箱中,用高锰酸钾和甲醛混和薰蒸
In the original production process, sometimes due to over-cultivation of culture materials, improper pH, lack of nutrients and other reasons, resulting in slow mycelium bacteria, or even eat the phenomenon of material, if allowed to develop, will inevitably produce bad bacteria, rework Will cause serious losses. In order to solve this problem, especially to the mushroom friends introduce a method of adding fluid. (A) the preparation of replenishment liquid If the original species just because of lack of water, just fill with tap water or clean water ponds can be; if the pH is too low, you can add clear lime water or add a small amount of NaOH, the solution should be less than alkaline pH9; If the pH value is too high, you can add the right amount of hydrochloric acid, pH value should be greater than 5; what nutritional supplement. (B) of the replenishment solution will replenish liquid Sheng large pots or other suitable container, put spoon, cover kraft paper or pressure plastic, rubber band or rope tied, cover, placed in a pressure cooker , Sterilized at a pressure of 1.4 kg / cm ~ 2 for 10 to 20 minutes, or sterilized for 30 to 40 minutes under atmospheric pressure. Cool to below 30 ℃ stand-by. (C) the addition of liquid The original species, replenishment, tweezers, alcohol lights, matches, etc. into the inoculation box, with potassium permanganate and formaldehyde fumigation