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目的了解慢性ＨＢＶ感染者外周血单核细胞（ＰＤＭＣ）中ＣＤ４~＋Ｔ细胞内ＩＬ－４和ＩＦＮ－γ的表达情况，以测定Ｔｈ０／Ｔｈ１／Ｔｈ２细胞的百分数，探明Ｔｈ１、Ｔｈ２细胞在慢性ＨＢＶ感染中的作用。方法常规分离ＰＢＭＣ，在ＰＭＡ、ｉｏｎｏｍｙｃｉｎ、莫能菌素的刺激下，采用流式细胞检测（ＦＡＣＳ）对慢性ＨＢＶ感染者ＰＢＭＣ中ＣＤ４~＋Ｔ细胞内ＩＬ－４和ＩＦＮ－γ表达进行分析。结果正常对照组，２．３％－１８.６％的ＣＤ４~＋Ｔ细胞为Ｔｈ１细胞，０.９％－９．２％为Ｔｈ２细胞；在慢性ＨＢＶ感染者外周血单个ＣＤ４~＋Ｔ细胞中，以Ｔｈ０细胞居多：而Ｔｈ１细胞则随着慢性乙型肝炎肝脏炎症活动的加剧而明显增多，在炎症活动明显的慢性乙型肝炎患者中，Ｔｈ１细胞百分数明显高于炎症活动呈静止状态的慢性乙型肝炎；Ｔｈ２细胞在慢性ＨＢＶ感染的不同阶段则较为恒定，但明显高于对照组。结论Ｔｈ１细胞与炎症活动呈正相关，Ｔｈ２细胞可能与ＨＢＶ感染的慢性化相关联。
Objective To investigate the expression of IL-4 and IFN-γ in CD4 ~ + T cells in peripheral blood mononuclear cells (PDMC) from patients with chronic HBV infection and to determine the percentage of Th0 / Th1 / Th2 cells. The role of HBV infection. Methods PBMCs were isolated routinely and stimulated by PMA, ionomycin and monensin. The expression of IL-4 and IFN-γ in CD4 ~ + T cells in PBMC of patients with chronic HBV infection were analyzed by flow cytometry (FACS). Results In the normal control group, 2.3% -18.6% of CD4 ~ + T cells were Th1 cells and 0.9% -9.2% were Th2 cells. In peripheral CD4 + T cells of chronic HBV infected patients, The majority of Th0 cells: while Th1 cells with chronic hepatitis B liver inflammatory activity increased significantly increased in patients with chronic inflammation of chronic hepatitis B, the percentage of Th1 cells was significantly higher than the inflammatory activity was at a standstill Chronic B Hepatitis; Th2 cells in the different stages of chronic HBV infection is relatively constant, but significantly higher than the control group. Conclusion There is a positive correlation between Th1 cells and inflammatory activity, Th2 cells may be associated with chronic HBV infection.