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目的探讨吴茱萸碱(evodiamine,EVO)刺激的负载脊髓匀浆蛋白(homogenate protein of spinal cord,hp)树突状细胞(hpDCevo)对小鼠脊髓损伤(spinal cord injury,SCI)的促修复作用及其机制。方法SCI后24 h给予治疗,随机分6组:分别腹腔注射PBS、EVO、未成熟DC(DCs)、经EVO刺激的DCs(DCevo)、负载hp的DC(hpDC)、hpDCevo。利用BMS功能评分及组织病理学方法,观察hpDCevo对小鼠脊髓损伤神经功能恢复和局部瘢痕等病理学改变的影响。ELISA法测定SCI损伤局部组织和T细胞培养上清中的脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、神经营养因子-3(Neurotrophin-3,NT-3)、白介素-12(interleukin-12,IL-12)、粒-巨噬细胞集落刺激因子(granulocyte-macrophage colonystimulating factor,GM-CSF)的含量。结果注射后第84天,hpDCevo组BMS评分明显高于hpDC组和其余各组(P<0.05)。组织病理学结果显示,hpDCevo组较其他组空洞和瘢痕缩小。损伤局部组织中的BDNF、NT-3、GM-CSF、IL-12含量为hpDCevo组高于hpDC组和其余各组(P<0.05);T细胞培养上清中的BDNF、GM-CSF为hpDCevo组高于hpDC组和其余各组(P<0.05)。结论hpDCevo对小鼠SCI修复有促进作用,其机制可能是通过上调损伤局部的BDNF、NT-3、GM-CSF、IL-12来实现的。
Objective To investigate the effects of evodiamine (EVO) stimulated homogenate protein of spinal cord (hp) dendritic cells (hpDCevo) on spinal cord injury (SCI) in mice. mechanism. Methods The patients were treated 24 hours after SCI and randomly divided into 6 groups: intraperitoneal injection of PBS, EVO, immature DCs (DCs), EVO-stimulated DCs (DCevo), hp-loaded DCs (hpDC), and hpDCevo. Using BMS function scores and histopathological methods, we observed the effect of hpDCevo on the recovery of spinal cord injury and pathological changes such as local scars. ELISA was used to determine brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and interleukin-12 in the local tissue of SCI injury and T cell culture supernatant. Interleukin-12, IL-12), granulocyte-macrophage colony stimulating factor (GM-CSF) content. Results On the 84th day after injection, the BMS score of hpDCevo group was significantly higher than that of hpDC group and the other groups (P<0.05). Histopathological results showed that the cavities in the hpDCevo group were smaller than those in the other groups. The contents of BDNF, NT-3, GM-CSF and IL-12 in the injured tissues were higher in the hpDCevo group than in the hpDC group and other groups (P<0.05); the BDNF and GM-CSF in the T cell culture supernatant were hpDCevo The group was higher than the hpDC group and the remaining groups (P<0.05). Conclusion hpDCevo can promote the repair of SCI in mice. The mechanism may be through up-regulation of BDNF, NT-3, GM-CSF and IL-12.