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AIM To investigate the effects of nesfatin-1 on gastric function in obese rats. METHODS The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H+/K+-ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes. RESULTS Body weight, body length, and Lee’s index of the rats significantly increased in the high-fat dietinduced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels. In addition, the expression of H+/K+-ATPase decreased and moved in line with changes in gastric acid secretory capacity. Thisin vivo experiment revealed that intracerebroventricular injection of nesfatin-1, rather than intravenous injection, could suppress gastric function in obese rats. Moreover, its effect on the gastric emptying and gastric acid secretory capacity of rats is dose-dependent within a certain period of time.CONCLUSION Through this research, we provide a theoretical basis for further studies on nesfatin-1, a potential antiobesity drug.
AIM To investigate the effects of nesfatin-1 on gastric function in obese rats. METHODS The obese rat model was induced by a high-fat diet. The gastric emptying rate and gastric acid secretory capacity of the rats were determined after treatment with different drug concentrations of nesfatin-1 and administration routes. Based on this, the expression of H + / K + -ATPase was measured using RT-PCR and western blot to preliminarily explore the mechanism of gastric acid secretion changes. RESULTS Body weight, body length, and Lee’s index of the rats significantly increased in the high-fat dietinduced obese rat model. Two hours after lateral intracerebroventricular injection of nesfatin-1, the gastric emptying rate and gastric acid secretory capacity of rats decreased. Four hours after injection, both were restored to normal levels . In addition, the expression of H + / K + -ATPase decreased and moved in line with changes in gastric acid secretory capacity. Thisin vivo experiment revealed that intrace Moreover, its effect on the gastric emptying and gastric acid secretory capacity of the rats is dose-dependent within a certain period of time. CONCLUSION Through this research , we provide a theoretical basis for further studies on nesfatin-1, a potential antiobesity drug.