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Objective To study the correlation between TNF and nerve root pain,and try to find out its significance.Methods 45 cases who had cervical syndrome,lumbar disc protrusion and lumbar spine stenosis were studied .TNF in blood serum was determined with subsequence saturation liquid phase competitive radio- immunity,and the results were further statistically processed. Results TNF value in 45 cases was higher than normal value(P<0.01). T- check- out was made between pain degree,we gained P<0.01. Conclusion There is notable correlation between TNF and nerve root pain,which is benifit to guide clinical diagnosis and treatment .