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目的对婴儿肠道细菌及乳杆菌组成进行分析。方法以66例出生1~7d的婴儿为调查对象,用分子生物学的手段,借助肠道细菌DNA指纹图谱和16SrDNAPCR扩增克隆分类(RFLP)技术。结果婴儿肠道菌群随出生天数增加而变化;乳杆菌特异性引物对PCR扩增结果表明,出生4h后乳杆菌已在婴儿肠道内定植,且定植时间与分娩方式无关;克隆多样性研究则显示婴儿肠道乳杆菌优势菌群为植物乳杆菌(74%)、嗜酸乳杆菌(14%)和约氏乳杆菌(12%)。结论婴儿肠道中的优势乳杆菌主要为植物乳杆菌。
Objective To analyze the composition of intestinal bacteria and lactobacilli in infants. Methods Sixty-six infants born 1 to 7 days old were enrolled in this study. Molecular biology was used to detect the bacterial DNA fingerprinting and 16S rDNA PCR amplification clonal classification (RFLP). Results The intestinal microflora of infants varied with the number of days of birth. The results of PCR amplification of lactobacilli-specific primers showed that lactobacilli were colonized in the intestine 4 h after birth, and the colonization time was independent of the mode of delivery. The dominant flora of Lactobacillus infantum showed Lactobacillus plantarum (74%), Lactobacillus acidophilus (14%) and Lactobacillus johnsonii (12%). Conclusion The dominant Lactobacillus in infant intestine is mainly Lactobacillus plantarum.