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研究病毒感染时宿主细胞mRNA转录调控的改变,对了解病毒与宿主相互作用关系,寻找抗病毒治疗的靶位点具有指导意义。虽然已有文献对一些病毒感染后引起细胞基因表达的改变作了清晰的描述,但目前仍不清楚细胞基因表达改变与病毒复制、基因表达和宿主细胞抗病毒反应等的关系。本文作者对此进行了探索。首先使用了一套含有12 626人cDNA位点的微点阵系统,对人巨细胞病毒(HCMV)感染人双倍染色体成纤维细胞后13个时间点(0.5、1、4、6、8、10、
Studying the change of transcriptional regulation of host cell mRNA during virus infection is instructive in understanding the relationship between virus and host and finding the target site of antiviral therapy. Although some literature has clearly described the changes of cell gene expression caused by some virus infections, it is still unclear whether the changes of cell gene expression are related to virus replication, gene expression and host cell antiviral response. The author of this article has explored. First, a set of 12 626 human cDNA microarray systems were used to detect human HCMV infection at 13 time points (0.5, 1, 4, 6, 8, 10,