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目的了解重庆地区博尔纳病病毒(Borna disease virus,BDV)基因特征及其种系发生。方法采用荧光定量巢式实时逆转录聚合酶链反应(fluorescence quantitative nested RT-PCR,FQ-nRT-PCR)检测健康牛、山羊、猪各50例以及病毒性脑炎(viral encephalitis,VE)患者20例外周血单核细胞(peripheral blood mononuclear cells,PBMCs)BDV p24片段,检出阳性序列和前期检出的5例重庆地区山羊和神经精神患者阳性BDV p24基因序列,与GenBank中5个国家7个动物种属33例BDV p24基因序列进行比对,分析其核苷酸和氨基酸序列同源性,重建基因系统发生树。结果10例BDV p24核苷酸序列一部分(1例VE患者)形成重庆独立支系,另一部分(7例动物和2例VE患者)汇聚至德国-瑞士-奥地利-日本-重庆混合支系。与BDV标准病毒株Strain V、H1766及He/80比对,9例核苷酸和氨基酸序列同源相似性分别为94%～100%和82%～100%,1例为95%～98%和89%～93%。结论重庆地区动物和人宿主可能存在地源性BDV独立株和源于外来疫病病原传入的流行株。
Objective To understand the gene and its phylogenetic relationship of Borna disease virus (BDV) in Chongqing. Methods FQ-nRT-PCR was used to detect the levels of 20 in healthy cow, goat and pig, and 20 patients with viral encephalitis (VE) by fluorescence quantitative nested RT-PCR Positive BDV p24 was detected in peripheral blood mononuclear cells (PBMCs) from peripheral blood mononuclear cells (PBMCs). Five positive BDV p24 gene sequences in Chongqing goat and neuropsychiatric patients were detected. The BDV p24 gene sequences of 33 species of animal species were aligned and their nucleotide and amino acid sequence homologies were analyzed to reconstruct the gene phylogenetic tree. Results A total of 10 cases of BDV p24 nucleotide sequence (1 patient with VE) formed an independent branch in Chongqing. The other part (7 animals and 2 patients with VE) converged on the German-Swiss-Austrian-Japanese-Chongqing mixed branch. Compared with the standard strains of Strain V, H1766 and He / 80 of BDV, the homologies of nine nucleotide and amino acid sequences were 94% -100% and 82% -100%, respectively, 95% -98% And 89% ~ 93%. Conclusion There may be indigenous BDV isolates and epidemic strains originating from foreign pathogens in animal and human hosts in Chongqing.