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建立一种采用高效液相色谱(HPLC)测定加工肉制品中四环素、土霉素和金霉素三种四环素族抗生素残留量的新方法。样品经体积分数0.5%的高氯酸溶液提取后,采用HLB固相萃取柱净化,用高效液相色谱C18柱分离,乙腈-甲醇-草酸作为流动相,流速为1.0 m L/min,梯度洗脱,紫外检测波长为275 nm,外标法峰面积定量。3种四环素质量浓度在10~100μg/L范围内均有较好的线性关系,回归系数R2均>0.99,方法检出限为20μg/kg,回收率范围为93.5%~97.4%,相对标准偏差小于15%(n=8)。该法简单,灵敏度高,重复性好,适用于动物源性食品中四环素族组份残留的测定。
A new method for the determination of tetracycline, oxytetracycline and chlortetracycline antibiotic residues in processed meat products by high performance liquid chromatography (HPLC) was established. The samples were extracted by 0.5% perchloric acid solution and purified by HLB solid phase extraction column. The samples were separated on a C18 column with acetonitrile-methanol-oxalic acid as mobile phase at a flow rate of 1.0 mL / min. Off, UV detection wavelength of 275 nm, external standard peak area quantitative. The linearity of the three tetracycline concentrations was in the range of 10-100 μg / L with R2> 0.99. The detection limit was 20 μg / kg and the recovery range was 93.5% -97.4%. The relative standard deviations Less than 15% (n = 8). The method is simple, sensitive and reproducible, and is suitable for the determination of tetracycline residues in animal-derived foods.