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目的:研究早期应用米诺环素(MINO)对不完全脊髓损伤大鼠神经功能修复的影响。方法:112只大鼠随机分为4组,假手术组(SHAM组)、损伤组(SCI组)、米诺环素低剂量治疗组、米诺环素高剂量治疗组,每组28只,SHAM组仅作椎板切除术,SCI组及米诺环素低剂量组、米诺环素高剂量组用改良Allen’s法制作T10不完全脊髓损伤模型,MINO低剂量组在术后0.5,1 h及1～5 d分别给予腹腔注射米诺环素40 mg·kg-1,MINO高剂量组以同样方法注射米诺环素80 mg·kg-1,SHAM、SCI组在同样的时间点注射等量的生理盐水。每组大鼠随机抽出16只在术前及术后1,3,7,14,21,28 d进行BBB评分,其余大鼠在术后3,7,21 d分别处死取材,行病理形态学观察(HE染色)和胶质纤维酸性蛋白(GFAP)免疫组化测定。结果:术后7～28 d,MINO低剂量组、MINO高剂量组BBB评分明显高于SCI组(P<0.05);HE染色显示在病理学形态上MI-NO低剂量组、MINO高剂量组脊髓损伤较SCI组轻;且GFAP表达明显受到抑制,与SCI组比较有明显差异(P<0.05)。结论:早期应用米诺环素可以促进不完全脊髓损伤大鼠神经功能的修复。
AIM: To investigate the effect of early application of minocycline on neurological repair in rats with incomplete spinal cord injury. Methods: One hundred and twelve rats were randomly divided into 4 groups: sham operation group (SHAM group), injury group (SCI group), minocycline low dose treatment group and minocycline high dose treatment group (n = 28) SHAM group was only used for laminectomy, SCI group and minocycline low dose group, minocycline high dose group modified Allen’s method to make T10 incomplete spinal cord injury model, MINO low dose group after 0.5,1 h And 1 to 5 days were given intraperitoneal injection of minocycline 40 mg · kg-1, MINO high-dose group by the same method of minocycline 80 mg · kg-1, SHAM, SCI group at the same time point injection The amount of saline. Sixteen rabbits in each group were randomly divided into three groups: preoperative and postoperative 1, 3, 7, 14, 21 and 28 days, BBB scores were performed. The remaining rats were sacrificed on days 3, 7 and 21 after operation. Pathological morphology Observations (HE staining) and glial fibrillary acidic protein (GFAP) immunohistochemical determination. Results: BBB scores of MINO low dose group and MINO high dose group were significantly higher than those of SCI group (P <0.05) at 7-28 days postoperatively. The HE staining showed that the BBB score of MI low dose group, MINO high dose group Spinal cord injury was lighter than SCI group, and the expression of GFAP was significantly inhibited compared with SCI group (P <0.05). Conclusion: The early application of minocycline can promote the repair of nerve function in rats with incomplete spinal cord injury.