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在获得神经生长因子（ＮＧＦ）ＩｇＧ后，以其为第一抗体建立了ＮＧＦ免疫组织化学方法，并观察了成年小不组织中ＮＧＦ的定位和分布。在颌下腺内，ＮＣＦ主要定位于粒曲小管细胞及纹状导管细胞，而且雌、雄物之间ＮＧＦ样免疫反应存在明显差异。第一抗体吸收试验观察到颌下腺显示免疫反应阴性，提示该反应是ＮＧＦ特异性的。舌下腺内可见到反应阳性的散在的小导管细胞。输精管平滑肌和心房肌也显示免疫反应阳性。肾组织的观察表明，不仅在一些远曲小管细胞，而且在部分近曲小管细胞均发现ＮＧＰ样免疫反应。
NGF immunohistochemistry was established using NGF as the primary antibody after obtaining nerve growth factor (NGF) IgG, and the localization and distribution of NGF in adult small non-tissue tissues were observed. In the submandibular gland, NCF mainly locates in granule cells and striated duct cells, and NGF-like immunoreaction between the female and male has significant differences. The first antibody absorption test observed submandibular gland showed immunoreactivity negative, suggesting that the reaction is NGF-specific. Sublingual glands can be seen in the reaction-positive scattered small ductal cells. Vas deferens smooth muscle and atrial muscle also showed immunoreactivity. The observation of kidney tissue showed that NGP-like immunoreactivity was found not only in some distal convoluted tubule cells but also in some proximal convoluted tubule cells.