槟榔种子提取物对离体大鼠主动脉的内皮依赖性血管舒张作用

• 作 者: 李宗友
• 出 处: 国外医学(中医中药分册)
• 发表日期: 1998年05期
• 关键词: 血管舒张作用 提取物 离体大鼠 内皮依赖性 catechu Areca 甲醇浓度 柱层析 表儿茶素 槟榔碱

试验材料:槟榔(Areca catechu)种子以沸水提取50分钟,提取液经冷冻干燥后得槟榔粗提取物(粉末,ASE)。另取部分粉末溶于水中,经Sephadex LH-20柱层析,以水和逐渐增加甲醇浓度(0％～30％)的洗脱液进行洗脱,水洗脱得非酚类成分(139.6mg),0％～30％甲醇洗脱得低分子量的酚性组分(9.0mg),甲醇洗脱得鞣质组分的混合物。

Test material: Areca catechu seeds were extracted with boiling water for 50 minutes and the extract was freeze-dried to obtain the betel nut crude extract (powder, ASE). Another portion of the powder was dissolved in water and subjected to Sephadex LH-20 column chromatography. The elution was carried out with water and an eluent that gradually increased the concentration of methanol (0% to 30%), and the water was eluted to give a non-phenolic component (139.6 mg). ), 0% to 30% methanol eluted to give a low molecular weight phenolic component (9.0 mg), which was eluted with methanol to give a mixture of tannin components.