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目的:研究卵巢癌细胞株SKOV3中ERβ表达对细胞增殖凋亡的影响。方法:MTT法检测卵巢癌细胞暴露于17β-E2不同浓度及不同时间的细胞增殖率。用特异性雌激素受体激动剂DPN联合17β-E2或单独使用DPN处理细胞,RT-PCR法检测ERβmRNA、ERK1/2 mRNA及caspase-3 mRNA表达,蛋白免疫印记法检测ERβ和p-ERK1/2蛋白表达。结果:10-6mol/L 17β-E2作用60min时,细胞的增殖活性最强(5.7±0.23),与对照组相比(3.5±0.45),差异有统计学意义(P<0.05)。ERβ在卵巢癌中低表达。随着DPN作用时间延长及浓度增加,ERβmRNA、caspase-3 mRNA表达逐渐增加(P<0.05),ERK2 mRNA表达逐渐降低,与对照组比较差异有统计学意义(P<0.05)。随着DPN作用时间延长及浓度的增加,ERβ蛋白表达逐渐增加,p-ERK1/2蛋白表达逐渐降低,与对照组比较差异有统计学意义(P<0.05)。结论:ERβ在卵巢癌中低表达,上调ERβ表达能抑制卵巢癌细胞的增殖,同时促进细胞的凋亡。
Objective: To study the effect of ERβ expression on proliferation and apoptosis of ovarian cancer cell line SKOV3. Methods: MTT assay was used to detect the proliferation of ovarian cancer cells exposed to different concentrations of 17β-E2 at different times. The expression of ERβ mRNA, ERK1 / 2 mRNA and caspase-3 mRNA were detected by RT-PCR with specific estrogen receptor agonist DPN combined with 17β-E2 or DPN alone. The expressions of ERβ and p-ERK1 / 2 protein expression. Results: The proliferative activity of 10-6mol / L 17β-E2 was the strongest (5.7 ± 0.23) at 60min compared with that of the control group (3.5 ± 0.45), the difference was statistically significant (P <0.05). ERβ is overexpressed in ovarian cancer. The expression of ERβmRNA and caspase-3 mRNA gradually increased (P <0.05) and the expression of ERK2 mRNA gradually decreased with the prolongation of DPN concentration and the increase of the concentration of ERK2 mRNA. The difference was statistically significant (P <0.05). With the prolongation of DPN time and the increase of concentration, the expression of ERβprotein gradually increased and the expression of p-ERK1 / 2 protein gradually decreased, which was significantly different from the control group (P <0.05). Conclusion: ERβ is overexpressed in ovarian cancer. Upregulation of ERβ expression can inhibit the proliferation of ovarian cancer cells and promote the apoptosis of ovarian cancer cells.