论文部分内容阅读
目的探讨献血者血液采用ELISA法和NAT联合检测降低经血传播乙型肝炎、丙型肝炎及艾滋病的效果。方法选取本站2015年4月~2016年12月采集的献血者血液样本72110例作为研究材料,所有样本均应用两种不同厂家试剂进行ALT、HBs Ag、抗-HCV、抗-HIV和抗-TP平行检测,同时对献血者样本进行NAT检测。结果在72 110例献血者中603例(0.84%)血清学HBs Ag、抗-HCV、抗-HIV、抗-TP检测不合格;71507例血清学检测合格献血者中检出HBV-DNA阳性49例(0.07%),未检出HCV-RNA和HIV-RNA阳性样本;在152例ELISA法HBs Ag 0.7≤S/CO<1.0样本和1.0≤S/CO≤3.0样本中检出13例HBV-DNA阳性,HBs Ag 1.0≤S/CO≤3.0样本NAT阳性检出率高于0.7≤S/CO<1.0样本,差异有统计学意义(P<0.05)。结论献血者血液样本采用ELISA法和NAT联合检测可降低经输血传播感染性疾病的风险,提高血液质量和输血安全。
Objective To study the effect of reducing the blood transfusion of Hepatitis B, Hepatitis C and AIDS by the combined detection of ELISA and NAT in the blood of blood donors. Methods A total of 72,110 blood samples collected from April 2015 to December 2016 in our hospital were selected as materials for the study. ALT, HBsAg, anti-HCV, anti-HIV and anti- TP parallel detection, while blood donor samples for NAT detection. RESULTS: Serum HBsAg, anti-HCV, anti-HIV and anti-TP were not detected in 603 out of 72 110 donors (0.84%). HBV-DNA was positive in 71 507 seropositive blood donors (0.07%), no positive samples of HCV-RNA and HIV-RNA were detected. Of 152 ELISA samples with HBsAg 0.7≤S / CO <1.0 and 1.0≤S / CO≤3.0, DNA positive, the positive detection rate of NAT in HBsAg 1.0≤S / CO≤3.0 was higher than 0.7≤S / CO <1.0, the difference was statistically significant (P <0.05). Conclusion Blood samples of blood donors using ELISA and NAT combined detection can reduce the risk of transfusion transmitted infectious diseases, improve blood quality and blood transfusion safety.