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目的研究椒蒿挥发油对自身免疫性睾丸炎小鼠CD4+CD25+调节性T细胞的影响。方法雄性BALB/c小鼠80只,随机分为4组(生理盐水组、模型组、橄榄油组、椒蒿组),采用睾丸内注射冰醋酸的方法造模,各组都在手术前3天开始灌胃,手术后继续灌胃,共连续灌胃24d(盐水组只灌胃,不手术)。分别于术后3周、9周取外周血,通过流式细胞仪对CD4+CD25+调节性T细胞进行检测。结果术后3周,模型组、橄榄油组、椒蒿组小鼠外周血CD4+CD25+调节性T细胞百分比均低于生理盐水组,差异有统计学意义(P<0.05),而此3组之间差异无统计学意义(P>0.05)。术后9周模型组与橄榄油组小鼠外周血CD4+CD25+调节性T细胞百分比低于生理盐水组,差异有统计学意义(P<0.05),椒蒿组与生理盐水组比较差异无统计学意义(P>0.05)。结论椒蒿能使自身免疫性睾丸炎小鼠CD4+CD25+调节性T细胞百分比提高,后者可发挥免疫调节作用。
Objective To study the effects of volatile oil of Artemisia annua L. on CD4 + CD25 + regulatory T cells in mice with autoimmune orchitis. Methods Eighty male BALB / c mice were randomly divided into 4 groups (normal saline group, model group, olive oil group, Artemisia annua L.). The rats were injected intralesional testicular injection of glacial acetic acid, Days began gavage, gavage continued after surgery, a total of continuous gavage 24d (saline group only gavage, no surgery). Peripheral blood was taken at 3 weeks and 9 weeks after operation, respectively. CD4 + CD25 + regulatory T cells were detected by flow cytometry. Results The percentages of CD4 + CD25 + regulatory T cells in the model group, olive oil group and Artemisia annua L. group were lower than those in the saline group three weeks after operation (P <0.05) The difference was not statistically significant (P> 0.05). The percentages of CD4 + CD25 + regulatory T cells in peripheral blood of model group and olive oil group at 9 weeks after operation were lower than those of saline group (P <0.05), there was no significant difference between the control group and the saline group Significance (P> 0.05). Conclusion Artemisia canadum can increase the percentage of CD4 + CD25 + regulatory T cells in mice with autoimmune orchitis, which can play an immunomodulatory role.