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目的研究树鼩血栓性脑缺血动物模型的MRI表现,探讨其动态演变规律。方法采用3.0 T MR在缺血后4 h、24 h、48 h及72 h对树鼩脑部行MRI,观察各时间点MRI表现,测量和计算出各时间点扩散加权成像(DWI)、T2WI异常信号体积;分别应用HE染色、红四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色技术显示脑缺血后不同时间点病理改变和皮层梗死体积的变化。结果缺血后4 h MRI可见信号改变,T2WI为高信号,T1WI为低信号,DWI为高信号,增强扫描可见强化;TTC染色所测体积与MRI体积比较,4 h时DWI及T2WI所测体积大于TTC(P<0.05),病理显示周边为水肿带;缺血后4 h至24 h梗死灶体积增大明显(P<0.05);光镜示缺血后中心区神经元可见皱缩,脱失明显,出现嗜酸神经元。结论 MRI可动态反映树鼩血栓性脑缺血动物模型缺血后不同时间点的病理演变过程。
Objective To study the MRI findings of the animal model of tree shrew thrombotic ischemia and to explore its dynamic evolution. Methods MRI was performed on the brain of tree shrews at 4 h, 24 h, 48 h and 72 h after ischemia with 3.0 T MR. The MRI findings at various time points were observed. Diffusion-weighted imaging (DWI) and T2WI Abnormal signal volume. The changes of pathological changes and cortical infarct volume at different time points after cerebral ischemia were observed by HE staining and TTC staining. Results After 4 h of ischemia, the signal changes were observed by MRI. The T2WI was high signal, T1WI was low signal, DWI was high signal, and enhanced scan showed enhancement. Compared with MRI volume, the volume measured by TTC staining showed that the volume measured by DWI and T2WI (P <0.05). Pathological examination showed that the surrounding area was edematous. The volume of infarction increased significantly from 4 h to 24 h after ischemia (P <0.05) Loss of sight, there eosinophil neurons. Conclusion MRI can dynamically reflect the pathological changes at different time points after ischemia in the animal model of tree shrews with cerebral ischemia.