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S-9 10μl/ml不减少肿瘤细胞集落形成。当其直接加入双层软琼脂系统上层,在环磷酰胺浓度为25,50,100和200μg/ml时,L_(1210)细胞的集落形成率分别为66,45,39和9％,HeLa细胞集落形成率为96,81,66和38％;甲基苄肼浓度为100,200和400μg/ml时,B_(16)细胞集落形成率为64,45和26％。不加S-9时,上述药物对各种细胞均不显示抑制作用。但人肝癌细胞株SMMC_(7721)虽不加S-9,在上述二药作用下集落形成率明显降低。
S-9 10 μl / ml did not reduce tumor cell colony formation. The colony formation rate of L 1210 cells were 66, 45, 39 and 9% when they were directly added to the upper layer of bilayer soft agar system at the concentrations of 25, 50, 100 and 200 μg / ml of cyclophosphamide. The colony formation of HeLa cells Rates were 96, 81, 66, and 38%. For Bcl-6 cells, the colony formation rates were 64, 45, and 26% at the concentrations of 100, 200 and 400 μg / ml respectively. Without S-9, the above drugs showed no inhibitory effect on various cells. However, the human hepatocellular carcinoma cell line SMMC_ (7721), although not adding S-9, significantly reduced the colony formation rate under the action of the two drugs.