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目的 比对分属于激活和抑制型中的不同核糖开关的调控功效,为实现基因电路的精准调控奠定基础.方法 构建不同核糖开关(addA与M6,TPP与btuB)调控的绿色荧光蛋白amcyan表达载体,通过荧光表达量、RT-qPCR分析在不同配体物浓度调控下的amcyan表达,与未含核糖开关载体的表达量进行比较,进行动态调控效能分析.结果 在addA与M6激活型核糖开关的调控下,随配体物浓度的增加,绿色荧光表达量增加,且相比M6核糖开关,addA核糖开关拥有更大的动态调控效能;相反,在TPP与btuB抑制型核糖开关的调控下,随配体物浓度的增加,绿色荧光表达量减少,但btuB核糖开关的动态调控效能略大于TPP核糖开关.结论 相同作用机制的不同核糖开关调控功效存在差异,拥有动态调控效能优势的激活型开关addA与抑制型开关btuB更适合在大肠杆菌中用于代谢精确调控与靶基因精准表达.“,”Objective To compare the regulation effects of different activated and inhibitory riboswitches , and to facilitate the precise regulation of gene circuits .Methods A green fluorescent protein amcyan expression vector regulated by different riboswitches (addA, M6, TPP and btuB) was constructed, and the expression level of amcyan under different ligand concentrations was analyzed by RT-qPCR and relative fluorescence intensity , and then compared with the expression level of a vector without any riboswitch .The dynamic control performance was analyzed .Results Under the control of addA and M6 activated riboswitches , the expression of green fluorescent protein increased with ligand concentrations , and addA riboswitch had more dynamic regulatory effect than M 6 riboswitch.However, under the control of TPP and btuB inhibitory riboswitches , the expression of green fluorescence decreased with the increase in ligand concentrations , and the dynamic regulation of btuB riboswitch was slightly greater than that of TPP riboswitch .Conclusion The regulation efficacy of different riboswitches which have the same mechanism varies .Activated riboswitch addA and inhibitory riboswitch btuB with dynamic regulation and control advantages are more suitable for precise metabolism regulation and target gene expression in Escherichia coli.