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目的了解深圳市无偿献血者乙肝病毒感染情况,分析其血清学和分子生物学特征。方法将收集到的确证HBs Ag(+)无偿献血者标本分为/NAT(+)和NAT(-)两组,进行乙肝两对半检测以及病毒核酸大容量提取,采用巢氏PCR方法扩增BCP/PC和S区基因序列,对所得的序列进行分析。结果在42 297份血液中235份HBs Ag确证为阳性的标本,HBs Ag阳性率为0.56%,其中NAT(+)162份,占总数68.9%,NAT(-)为73份,占31.1%,血清学模式以HBs Ag(+)、抗-HBe(+)、抗-HBc(+)为主,占69.78%,检出10例抗-HBc(-)标本,占总数的4.25%。在可测序166标本中,B基因型所占88%(146/166),C基因型占11.4%(19/166),D基因型在HBs Ag(+)/NAT(+)出现1例。结论 NAT检测与HBs Ag检测方法均只能检出部分乙肝感染献血者,血液检测必须结合两种检测方法来保证血液安全。数据显示有必要应用灵敏度更高的NAT检测方法。
Objective To understand the status of hepatitis B virus infection in unpaid blood donors in Shenzhen and to analyze its serological and molecular biological characteristics. Methods The positive HBsAg (+) blood donors collected were divided into two groups, NAT / NAT (+) and NAT (-). HBV pairs were tested for half and two pairs of HBsAg, BCP / PC and S region gene sequence, the resulting sequence analysis. Results Of the 42 297 blood samples, 235 were positive for HBsAg, the positive rate of HBs Ag was 0.56%, of which 162 were NAT (+), accounting for 68.9% of the total, and 73 were NAT (-), accounting for 31.1% Serological patterns of HBs Ag (+), anti-HBe (+) and anti-HBc (+) accounted for 69.78%. Ten anti-HBc (-) samples were detected, accounting for 4.25% of the total. In the sequenced 166 specimens, B genotype accounted for 88% (146/166), C genotype 11.4% (19/166), D genotype HBs Ag (+) / NAT (+) in 1 case. Conclusion Both NAT and HBsAg detection methods can only detect part of HBV infected blood donors. Blood tests must be combined with two test methods to ensure blood safety. The data shows that it is necessary to apply the more sensitive NAT detection method.