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目的探讨表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)乙酰化衍生物(AcEGCG)是否对人肝癌细胞SMMC-7721有细胞毒增敏作用。方法用四甲基偶氮噻唑蓝(MTT)法检测EGCG和AcEGCG分别联合柔红霉素(DNR)对人肝癌细胞SMMC-7721的毒性作用,实验设35、100、160μmol/L EGCG及AcEGCG组、35、100、160μmol/L EGCG及AcEGCG分别联合柔红霉素DNR(0.9μmol/L)组,金正均法计算q值,判断两药相互作用。结果 35、100μmol/L AcEGCG联合DNR对人肝癌细胞SMMC-7721的细胞毒增敏作用较相同剂量的EGCG强(67.0%对48.1%,78.9%对65.0%),差异具有统计学意义(P<0.05),160μmol/L AcEGCG联合DNR对人肝癌细胞SMMC-7721的细胞毒增敏作用比EGCG稍弱(P>0.05)。结论在一定剂量范围内,AcEGCG联合DNR对人肝癌细胞SMMC-7721的细胞毒增敏作用较EGCG强。
Objective To investigate whether the epigallocatechin gallate (ACGCG) acetylated derivative (AcEGCG) has a cytotoxic effect on human hepatocellular carcinoma cell line SMMC-7721. Methods The toxic effects of EGCG and AcEGCG combined with daunorubicin (DNR) on human hepatocellular carcinoma cell line SMMC-7721 were detected by MTT assay. The effects of 35, 100, 160μmol / L EGCG and AcEGCG , 35,100,160μmol / L EGCG and AcEGCG respectively combined with daunorubicin DNR (0.9μmol / L) group, qingzheng average value calculated q value, to determine the interaction between the two drugs. Results The cytotoxic effect of 35,100μmol / L AcEGCG combined with DNR on human hepatocellular carcinoma cells SMMC-7721 was significantly higher than that of the same dose of EGCG (67.0% vs 48.1%, 78.9% vs 65.0%, P < 0.05). The cytotoxic effect of AcEGCG combined with DNR at 160μmol / L on human hepatoma SMMC-7721 cells was weaker than that of EGCG (P> 0.05). Conclusion AcEGCG combined with DNR has a stronger cytotoxic effect on human hepatocellular carcinoma cell SMMC-7721 than EGCG in a certain dose range.