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以温度和播种深度为试验因子的种子发芽试验,可以在田间、温室及生长室进行,但由于时间及设备所限,只能设置较少的试验处理.利用改良后的单向温度(热量)梯度盘,可在不同温度下对不同播种深度的种子进行发芽试验.1958年,Halldal和French开始采用灯光交叉温度梯度设计,进行光照及温度二因素对藻类的萌发效应实验.此后,人们普遍采用温度梯度盘进行种子发芽及其他试验,并不断对梯度盘加以改进.例如Thompson(1970年)用“温度梯度栅”探讨了温度对野生植物种子的发芽效应与其对自身生长环境适应性的关系;Hendricks等(1976年)应用温度梯度盘,研究了不同温度下种子发芽性能与细胞膜氨基酸的渗漏量之关系.
Seed germination test with temperature and sowing depth as test factors can be carried out in field, greenhouse and growth chamber, but due to the limited time and equipment, only a few test treatments can be set up.Using the improved unidirectional temperature (heat) Gradient disk can germinate seeds at different sowing depths at different temperatures. In 1958, Halldal and French began to use light crossing temperature gradient design to experiment on germination of algae with two factors of light and temperature. Since then, it has been widely used For example, Thompson (1970) used “Temperature Gradient Grids” to investigate the effect of temperature on the germination of wild plant seeds and their adaptability to their own environment of growth Hendricks et al. (1976) studied the relationship between seed germination and membrane amino acid leakage at different temperatures using a temperature gradient disk.