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目的:探讨不同低氧运动模式对大鼠免疫功能的影响。方法:30只SD大鼠随机分为常氧运动组(对照组)、高住高练组和高住低练组,对照组大鼠每天在常氧环境下生活23 h,训练1 h;高住高练组每天在低氧环境生活23 h,训练1 h;高住低练组每天在低氧环境生活12 h,常氧环境下生活11 h,训练1 h。全血分析仪分析全血指标,流式细胞仪检测CD4~+和CD8~+占有的百分比。结果:与高住低练组及对照组比较,高住高练组大鼠血液红细胞计数(RBC)和血红蛋白水平(Hb)升高,CD4~+T细胞百分数降低,CD8~+T细胞百分数升高,CD4~+/CD8~+比值下降,差异均具有统计学意义(P<0.05)。对照组大鼠、高住高练组和高住低练组ELISPOT斑点数依次为(230.0±10.6)/2×10~5个脾细胞、(56.5±6.8)/2×10~5个脾细胞和(89.3±9.8)/2×10~5个脾细胞,差异有统计学意义(P<0.05)。结论:低氧运动可以提高机体氧运输能力,但是可能会损害机体免疫功能。
Objective: To investigate the effects of different hypoxic exercise modes on immune function in rats. Methods: Thirty SD rats were randomly divided into normoxia group (control group), high-living training group and high-living-low training group. The rats in control group were exposed to normoxia for 23 hours every day for 1 h. Living high training group daily life in hypoxia for 23 h, training 1 h; high-living low training group daily life in hypoxia 12 h, 11 h in normoxic environment, training 1 h. Whole blood analyzer analysis of whole blood indicators, flow cytometry CD4 ~ + and CD8 ~ occupy the percentage. Results: Compared with the high living training group and the control group, the blood red blood cell count (RBC) and hemoglobin level (Hb) increased, the percentage of CD4 ~ + T cells decreased and the percentage of CD8 + T cells increased High, CD4 ~ + / CD8 ~ + ratio decreased, the difference was statistically significant (P <0.05). The number of ELISPOT spots in control group, living-high training group and high-living-training group were (230.0 ± 10.6) / 2 × 10-5 splenocytes, (56.5 ± 6.8) / 2 × 10-5 splenocytes And (89.3 ± 9.8) / 2 × 10 ~ 5 splenocytes, the difference was statistically significant (P <0.05). Conclusion: Hypoxic exercise can improve oxygen transport capacity of the body, but may damage the body’s immune function.