血小板活化因子及其受体对颈髓损伤后细胞粘附分子表达的调控作用

• 作 者: 肖建如 曾华武 李医明 侯铁胜 赵定麟
• 机 构: 上海第二军医大学长征医院骨科
• 出 处: 上海医学
• 发表日期: 1998年09期
• 关键词: 颈髓损伤 血小板活化因子 细胞粘附分子 血管内皮细胞 鞘内注射 地高辛标记 原位杂交技术 假手术对照组 损伤组 原位杂交方法

目的：为了探讨血小板活化因子（ＰＡＦ）及其受体对颈髓损伤后颈髓血管内皮细胞细胞间粘附分子１ｍＲＮＡ（ＩＣＡＭ１ｍＲＮＡ）和内皮细胞白细胞粘附分子１ｍＲＮＡ（ＥＬＡＭ１ｍＲＮＡ）表达的影响。方法：通过Ａｌｅｎ’ｓ法造成鼠Ｃ６７挫伤，采用鞘内注射ＰＡＦ及静脉注射ＰＡＦ受体拮抗剂ＢＮ５２０２１，应用地高辛标记ｃＲＮＡ探针原位杂交技术检测颈髓损伤后颈髓血管内皮细胞上述粘附分子的表达。结果：伤后６～１２小时颈髓血管内皮细胞ＩＣＡＭ１ｍＲＮＡ、ＥＬＡＭ１ｍＲＮＡ表达呈不同程度的增加；鞘内注射ＰＡＦ可使伤后血管内皮细胞ＩＣＡＭ１ｍＲＮＡ、ＥＬＡＭ１ｍＲＮＡ表达明显增加，ＰＡＦ受体拮抗剂可抑制ＩＣＡＭ１ｍＲＮＡ、ＥＬＡＭ１ｍＲＮＡ表达。结论：ＰＡＦ通过增加伤后颈髓血管内皮细胞粘附分子的表达是导致血脊髓屏障损害的重要分子基础。ＰＡＦ受体对伤后血管内皮细胞细胞粘附分子的表达具有调控作用。

Objective: To investigate the effect of platelet activating factor (PAF) and its receptor on the expression of intercellular adhesion molecule1 mRNA (ICAM1 mRNA) and endothelial cell leukocyte adhesion molecule1 mRNA (ELAM1 mRNA) in cervical spinal cord endothelial cells The impact of expression. Methods: C6.7 contusion was induced by Alen’s method. PAF was injected intrathecally and PA5 receptor antagonist BN52021 was injected intravenously. Digoxin-labeled cRNA probe in situ hybridization was used to detect the changes of cervical intravascular endothelial Cell expression of the above adhesion molecules. Results: ICAM 1mRNA and ELAM-1mRNA expression in cervical vascular endothelial cells were increased in 6 ~ 12h after injury; intrathecal injection of PAF could significantly increase the expression of ICAM1mRNA and ELAM1mRNA in injured vascular endothelial cells, PAF Receptor antagonist can inhibit I  CAM  1 mRNA, ELAM  1 mRNA expression. CONCLUSION: PAF is an important molecular basis for the damage of blood-spinal cord barrier by increasing the expression of adhesion molecules in injured cervical spinal cord endothelial cells. PAF receptor plays a regulatory role in the expression of cell adhesion molecules in injured vascular endothelial cells.