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作者采用免疫组织化学及图象分析技术发现,经压力峰值178.5 dB(SPL)的冲击波一次暴露后第1日,豚鼠螺旋神经节(SG)细胞P物质免疫反应(SP-IR)阳性产物的光密度较对照组明显增高(P<0.01),暴露后第3日,光密度较对照组为低(P<0.05),第7日恢复正常.暴露后各圈之间SP-IR产物的光密度无明显差异(P>0.05).免疫透射电镜发现爆震后第1日,豚鼠SGI型细胞胞浆内SP-IR产物明显增多,并可见较多的直径50～70nm的清亮小囊泡,有的囊泡包绕线粒体形成环形结构.爆震后不同时间组豚鼠的ABR阈移与SP-IR产物光密度的变化率成正相关(r=0.7251,P<0.05).结果提示冲击波所致听力损伤与SG细胞内SP的变化有关.
Using immunohistochemistry and image analysis, we found that on day 1 of shock wave exposure at a peak pressure of 178.5 dB (SPL), the light of SP-IR positive product of spiral ganglion (SG) cells in guinea pigs (P <0.01), and the optical density was lower on the 3rd day after exposure than that of the control group (P <0.05), and returned to normal on the 7th day after exposure.The optical density of SP-IR products Immuno-transmission electron microscopy showed that the cytoplasmic SP-IR products of guinea pig SGI-type cells were significantly increased on the first day after detonation, and more clear small vesicles with diameters of 50-70 nm were found Of the vesicles around the mitochondria to form a ring structure.The ABR threshold shift of guinea pigs at different time after knocking was positively correlated with the rate of change of optical density of SP-IR product (r = 0.7251, P <0.05) It is related to the change of SP in SG cells.