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目的:通过测定大鼠急性心肌缺血/再灌注损伤模型中lrg表达水平与血浆降钙素基因相关肽(CGRP)、内皮素(ET-1)、肿瘤坏死因子-α(TNF-α)表达水平及心肌梗死面积的变化,探索内毒素对缺血/再灌注心肌保护的分子机制。方法:将30只SD大鼠随机分为单纯缺血/再灌注模型组、内毒素预处理组和手术对照组,每组10只。采用戊巴比妥钠(40 mg/kg)腹腔注射麻醉大鼠,采用穿线结扎左冠状动脉前降支制备大鼠心肌缺血/再灌注模型。采用免疫印迹法结合图像分析软件半定量计算lrg分子表达水平的动态变化,放射免疫法测定血浆CGRP、ET-1和TNF-α浓度,用氯化三苯四唑(TTC)染色法和计算机图像分析系统计算心肌梗死面积。结果:大鼠左冠状动脉前降支缺血/再灌注1.5 h内毒素预处理组血浆ET-1和血清TNF-α浓度较缺血/再灌注组显著降低,心肌梗死面积也显著缩小(P<0.01);而血浆CGRP浓度则显著增高(P<0.01)。结论:内毒素预处理可诱导lrg分子表达水平上调,并显著降低ET-1而增加CGRP浓度,减小心肌梗死面积,对急性心肌梗死后再灌注心肌产生一定的保护效应。
OBJECTIVE: To investigate the relationship between the expression of lrg and the levels of plasma calcitonin gene related peptide (CGRP), endothelin (ET-1) and tumor necrosis factor-α (TNF-α) in rats with acute myocardial ischemia / reperfusion injury Level and myocardial infarction area changes, to explore the molecular mechanism of endotoxin on ischemia / reperfusion myocardial protection. Methods: Thirty SD rats were randomly divided into simple ischemia / reperfusion model group, endotoxin pretreatment group and operation control group, with 10 rats in each group. The rats were anesthetized by pentobarbital sodium (40 mg / kg) intraperitoneally, and the model of myocardial ischemia / reperfusion was established by ligating left anterior descending coronary artery. Western blotting and image analysis software were used to semi-quantitatively calculate the dynamic changes of lrg molecule expression. Plasma CGRP, ET-1 and TNF-α concentrations were determined by radioimmunoassay. TTC staining and computer image Analysis system to calculate myocardial infarct size. Results: Compared with ischemia / reperfusion group, the concentrations of ET-1 and serum TNF-α in endotoxin pretreatment group and ischemia / reperfusion group were significantly decreased (P <0.01), while plasma CGRP concentration was significantly higher (P <0.01). Conclusion: Endotoxin preconditioning can induce the up-regulation of lrg molecule expression, decrease ET-1, increase CGRP concentration, decrease infarct size, and protect myocardium from reperfusion after acute myocardial infarction.