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目的 探讨同种输血对食管癌患者细胞因子生成的影响及相互关系。方法 食管癌患者手术常规输血18例 ,去白细胞输血 14例 ,采用生物素 亲和素系统测定技术检测患者围手术期常规输血和去白细胞输血后血清中IL 10、IFN γ和TNF α的浓度。结果 常规输血后第 1天与输血前相比血清中IL 10、IFN γ和TNF α浓度升高 ,且以IL 10、IFN γ变化尤为显著 ;输血后第 5天IFN γ和TNF α降低接近输血前水平 ,并明显低于去白细胞输血组 ,IL 10仍明显高于输血前水平。去白细胞输血后不同时间IL 10无显著性改变。结论 食管癌患者围手术期同种输血后血清中IFN γ和TNF α的降低与IL 10升高有关 ,而IL 10升高可能是患者同种输血后免疫抑制的最重要原因 ,输注去白细胞血液可减轻或去除这一作用。
Objective To investigate the effects of allogeneic blood transfusion on cytokine production in patients with esophageal cancer and its relationship. Methods The routine blood transfusions in 18 patients with esophageal cancer and the leukocyte transfusion in 14 patients were performed. The concentrations of IL-10, IFN-γ and TNF-α in serum after routine blood transfusion and leukocyte transfusion were detected by biotin-avidin system. Results The levels of IL-10, IFN-γ and TNF-α in serum were significantly higher than those before transfusion on the first day after routine blood transfusion, especially in IL 10 and IFNγ. On the fifth day after transfusion, the levels of IFN-γ and TNF- Before the level, and significantly lower than the leukocyte transfusion group, IL 10 is still significantly higher than the level before transfusion. IL 10 did not change significantly at different times after leukocyte transfusion. Conclusion The decrease of serum IFN-γ and TNF-α in patients with esophageal cancer during perioperative blood transfusion is related to the increase of IL-10, while the increase of IL-10 may be the most important reason of immunosuppression after allo-transfusion in patients with esophageal cancer. Infusion of leukocytes Blood can reduce or eliminate this effect.