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目的探讨布渣叶提取物对四氯化碳(CCl4)所致小鼠急性化学性肝损伤的保护作用。方法将50只小鼠随机分为五组(每组10只):正常对照组、CCl4模型组、布渣叶低剂量组(100mg/kg)、布渣叶中剂量组(200mg/kg)和布渣叶高剂量组(400mg/kg)。布渣叶低剂量组、中剂量组和高剂量组以不同浓度相同剂量的布渣叶提取物灌胃,正常对照组和CCl4模型组以等量的生理盐水灌胃,连续7d。第七天灌胃后2h,CCl4模型组、布渣叶低剂量组、中剂量组和高剂量组分别腹腔注射CCl4,建立CCl4所致小鼠急性化学性肝损害模型。12h后采血定量测定各组小鼠血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、谷胱甘肽过氧化物酶(GSH)的活性和总超氧化物歧化酶(T-SOD)活力,并观察肝组织病理学变化,以评价肝损害的程度和药物的保护作用。结果布渣叶提取物能明显降低CCl4所致急性肝损伤小鼠血清GPT、GSH水平,提高GSH活性及SOD活力,肝脏组织病理损伤得到明显改善,且呈明显的量效关系。结论布渣叶提取物对CCl4所致的急性化学性肝损伤有良好的保护作用。
Objective To investigate the protective effect of cloth residue extract on acute chemical liver injury induced by carbon tetrachloride (CCl4) in mice. Methods Fifty mice were randomly divided into five groups (n = 10): normal control group, CCl4 model group, low-dose cloth residue leaf group (100mg / kg), middle-dose group (200mg / kg) Residue high dose group (400mg / kg). The cloth residue leaves low dose group, medium dose group and high dose group were fed with different concentrations of the same dose of cloth residue extract, normal control group and CCl4 model group with the same amount of normal saline, continuous 7d. At 2h after the seventh day, the CCl4 model group, the low dose group, the medium dose group and the high dose group were intraperitoneally injected with CCl4 to establish acute chemical liver injury model induced by CCl4 in mice. Blood samples were collected for determination of GPT, GOT and GSH activities and total superoxide dismutase (T-SOD) activity after 12 h. And observe the histopathological changes of liver to evaluate the degree of liver damage and drug protective effect. Results Cloth leaf extract can significantly reduce the acute liver injury caused by CCl4 in mice serum GPT, GSH levels, increased GSH activity and SOD activity, liver tissue pathology was significantly improved, and showed a significant dose-effect relationship. Conclusion Cloth leaf extract has a good protective effect on CCl4-induced acute chemical liver injury.