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目的:探讨玉葵清对糖基化终产物(AGEs)干预的人肾小球系膜细胞(HRMC)结缔组织生长因子(CTGF)mRNA及其蛋白表达的影响。方法:用中药血清药理学方法制备玉葵清含药血清(YKQ);用同一浓度YKQ(1.25%)和AGEs(200μg/mL)干预HRMC不同的时间(0、8、16、24、48、72h)以及用不同浓度的YKQ(0.313%、0.625%、1.25%)和AGEs(200μg/mL)干预HRMC48h,以无血清培养基(DMEM)和相应浓度的鼠血清(RS)为对照,用RT-PCR法和Western Blotting分别检测CTGF mRNA和蛋白的表达水平。结果:与0h相比,YKQ呈时间依赖减轻AGEs干预的HRMC CTGF mRNA和蛋白的表达(P<0.05)以及YKQ浓度在0.313%、0.625%和1.25%时,较对照组显著减轻CTGF mRNA和蛋白的表达(P<0.05)。结论:玉葵清能抑制AGEs干预的HRMC CTGF mRNA和蛋白的表达,对糖尿病肾病起一定的防治作用,此作用可能是其防治糖尿病肾病的机制之一。
Objective: To investigate the effect of Yacon on expression of connective tissue growth factor (CTGF) mRNA and protein in human glomerular mesangial cells (HRMC) interfered by advanced glycation end products (AGEs). Methods: YKQ was prepared by Chinese Herbal Serum Pharmacology. HRQMCs were treated with YKQ (1.25%) and AGEs (200μg / mL) for different time (0,8,16,24,48, 72h) and HR48 cells were treated with different concentrations of YKQ (0.313%, 0.625%, 1.25%) and AGEs (200μg / mL) for48h. Serum free medium (DMEM) and corresponding serum (RS) The expression of CTGF mRNA and protein were detected by PCR and Western Blotting respectively. Results: Compared with 0h, YKQ significantly delayed the expression of CTGF mRNA and protein (P <0.05) and the YKQ concentrations at 0.313%, 0.625% and 1.25% in a time-dependent manner significantly reduced the expression of CTGF mRNA and protein (P <0.05). CONCLUSION: Yacon can inhibit the expression of HRGF CTGF mRNA and protein in AGEs-induced diabetic nephropathy, which may be one of the mechanisms of its prevention and treatment of diabetic nephropathy.