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为了研究T-2毒素对小鼠胚胎体外发育的影响,将40只6～8周龄妊娠4 d清洁级昆明小鼠处死,挑选形态良好的8-细胞胚胎。采用微滴培养法,将形态良好的8-细胞胚胎(10～15个/孔)分别移入0(溶剂对照)、10-9、10-8、10-7 mol/L的T-2毒素培养液(含5%人输卵管液+10%胎牛血清)中,记录囊胚形成数及囊胚脱带数,并计算囊胚形成率和囊胚脱带率。每个剂量设5个平行。结果显示,与溶剂对照组比较,T-2毒素浓度在10-8和10-7 mol/L时囊胚形成率较低,差异均有统计学意义(P<0.05);T-2毒素浓度在10-9、10-8和10-7 mol/L时,囊胚脱带率较低,差异均有统计学意义(P<0.05)。囊胚形成率和囊胚脱带率均随着T-2毒素浓度的升高呈下降趋势。提示T-2毒素在小鼠胚胎发育过程中具有毒性作用。
In order to study the effect of T-2 toxin on the in vitro development of mouse embryos, 40 clean Kunming mice of 6-8 weeks old at 4 days of gestation were sacrificed and morphologically selected 8-cell embryos were selected. The microtiter well culture method was used to transfer the well-formed 8-cell embryos (10-15 cells / well) into 0 (solvent control), 10-9, 10-8 and 10-7 mol / L T-2 toxin Fluid (containing 5% human fallopian tube fluid + 10% fetal bovine serum), the number of blastocyst formation and the number of blastocyst removal were recorded, and the blastocyst formation rate and blastocyst removal rate were calculated. Set 5 for each dose parallel. The results showed that the blastocyst formation rate of T-2 toxin at 10-8 and 10-7 mol / L was lower than that of the solvent control group (P <0.05). The concentration of T-2 toxin At 10-9, 10-8 and 10-7 mol / L, the rate of blastocyst removal was lower, the differences were statistically significant (P <0.05). The blastocyst formation rate and blastocyst debonding rate all decreased with the increase of T-2 toxin concentration. Tip T-2 toxin in mouse embryonic development has a toxic effect.