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为了解恩诺沙星对异育银鲫(Carassius auratus gibelio)的急性毒性及血液生化指标的影响,评价恩诺沙星对异育银鲫的安全性。本实验研究了恩诺沙星对异育银鲫的急性毒性,并观察了恩诺沙星在不同剂量下对异育银鲫血液生化指标的影响。实验结果表明,恩诺沙星对异育银鲫的半数致死剂量为1 949.84 mg/kg,安全剂量为194.98 mg/kg。当恩诺沙星以常规给药剂量(20 mg/kg)连续口灌异育银鲫30 d时,异育银鲫的血清总蛋白含量、谷丙转氨酶含量、谷草转氨酶、γ-谷氨酰转移酶含量与对照组没有显著变化;而当恩诺沙星以40 mg/kg、80 mg/kg、160 mg/kg、320 mg/kg的剂量连续口灌异育银鲫30 d时,异育银鲫的血清谷丙转氨酶含量较对照组分别增加了23%、30%、46%、86%(P<0.05),血清谷草转氨酶含量较对照组分别增加了37%、42%、69%、86%(P<0.05)。本研究确定了恩诺沙星对异育银鲫的安全剂量,证实了恩诺沙星引起异育银鲫出现肝功能失调的剂量范围,对恩诺沙星在异育银鲫养殖中的安全使用提供了科学依据,也证实常规给药剂量(20 mg/kg)的恩诺沙星不会导致异育银鲫出现肝损伤。
To evaluate the effect of enrofloxacin on the acute toxicity and blood biochemical parameters of Carassius auratus gibelio, the safety of enrofloxacin against allogynogenetic crucian carp (Carassius auratus gibelio) was evaluated. This study studied the acute toxicity of enrofloxacin to allogynogenetic crucian carp (Carassius auratus gibelio) and observed the effect of enrofloxacin on the biochemical parameters of allogynogenetic crucian carp at different doses. The experimental results showed that the median lethal dose of enrofloxacin for allogynogenetic crucian carp was 1 949.84 mg / kg and the safe dose was 194.98 mg / kg. When enrofloxacin was continuously dosed at different doses (20 mg / kg) for 30 d, the serum total protein, alanine aminotransferase, aspartate aminotransferase, γ-glutamyl Transfected enzyme content did not change significantly compared with that of the control group. When enrofloxacin was continuously dosed with 30 mg / kg, 80 mg / kg, 160 mg / kg and 320 mg / kg for 30 days, Compared with the control group, the content of serum alanine aminotransferase in Carassius auratus increased by 23%, 30%, 46%, 86% (P <0.05) and serum aspartate aminotransferase increased by 37%, 42%, 69% , 86% (P <0.05). In this study, the safety dose of enrofloxacin on allogynogenetic crucian carp (Carassius auratus gibelio) was determined and the dose range of enrofloxacin induced liver dysfunction was confirmed. The safety of enrofloxacin in the breeding of allogynogenetic crucian carp The use provided a scientific basis and also confirmed that enrofloxacin at a conventional dose (20 mg / kg) did not cause liver damage in C. crucian carp.