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目的 总结呼吸道合胞病毒(RSV)在其流行季节的亚型分布情况;探讨RSV亚型和临床疾病严重程度的相关性。方法 采用RT PCR 酶联夹心杂交法检测了2 0 0 2年1 0月至2 0 0 3年3月和2 0 0 3年1 0月至2 0 0 4年3月连续两年冬春季期间就诊于温州医学院附属儿童医院的2 0 2例急性下呼吸道感染的婴幼儿鼻咽部分泌物中RSV及其亚型;结合临床资料,对临床症状和体征进行疾病严重程度评分。结果 2 0 2例标本中RSV阳性共1 2 3例,阳性检出率为60 . 9% ;两个冬春季都以RSVA亚型为主,2 0 0 2～2 0 0 3年冬春季A亚型占RSV阳性标本中76 7% ,2 0 0 3～2 0 0 4年占82. 0 % ;通过对97例A亚型和2 6例B亚型病例临床资料的评估,发现B亚型引起的临床症状和体征更重(P <0. 0 5)。结论 RSV是引起冬春季婴幼儿急性下呼吸道感染的最主要的病原体;以A亚型为主,但B亚型引起的临床表现更严重。
Objective To summarize the subtype distribution of respiratory syncytial virus (RSV) in its epidemic season and to explore the correlation between RSV subtype and the severity of clinical disease. Methods RT PCR enzyme-linked sandwich hybridization was used to detect the growth of winter in spring and spring between February 10 and March 2003 and October 2003 to March 2004 Visits to RSV and its subtypes in nasopharyngeal secretions of infants with 202 cases of acute lower respiratory tract infection at the Children’s Hospital Affiliated to Wenzhou Medical College; Combined with clinical data, the severity of clinical symptoms and signs were scored. Results A total of 120 positive cases of RSV were found in 202 samples, with a positive rate of 60.9%. The two RSV subtypes were dominant in winter and spring, while the other two in spring and winter were A202 ~ 2003 Subtypes accounted for 76.7% of RSV positive samples and 82.0% of cases from 2003 to 2004. By evaluating the clinical data of 97 subtype A and 26 subtype B patients, it was found that subtype B Type caused more severe clinical symptoms and signs (P <0. 05). Conclusions RSV is the most important causative agent of acute lower respiratory tract infection in infants and young children in winter and spring. The main subtype A is RSV, but the clinical manifestation of subtype B is more serious.